What is it?
A new concept for single-shot volumetric fluorescence imaging using orthogonally polarized excitation lattices has been developed by Florian Strohl and Clemens Kaminski. Essentially two orthogonally, perpendicular or in a right angle, polarized - restricted to one direction - excitation lattices are placed with a phase shift - a shift in the waveform - of p between them. With non-iterative image reconstruction algorithm, meaning it does not occur continuously over a designated range, scientist are able to find patterns and develop theoretical framework for the method.
Why it matters?
This is important because it gives insight into information that could not be accessed before. Single-shot imaging gains access to missing cone information. Before, deconvolution - basically deciphering the incoming information - of wide-field fluorescence images provides guesses of spatial frequency. Scientists would be able to see fixed biological cells.
Read more at https://www.nature.com/articles/s41598-019-42743-4
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